BIOTECHNOLOGY- PRINCIPLES AND PROCESSES
1. Raju is searching blight resistant plants for his crossing experiments. Unfortunately no such varieties are available.
a. Suggest a process to create such plants.
b. Justify your suggested method. (2)
2. Rinku with a circular DNA contains sequence
5’ → GGAATTCC → 3’
3’ → CCTTAAGG → 5’
She wishes to add a new segment of DNA into it.
a. Identify the technology she planned.
b. Suggest the specific enzyme to make a cut in the DNA with above sequence.
c. Name the category of enzyme you suggested.
d. How this enzyme identifies the sequence?
e. Draw the cut ends of the DNA with sequence.
Rashid isolated a natural plasmid from a bacterium and planning to facilitate cloning.
a. What are the minimum requirements for considering the isolated plasmid as a vector?
b. How he identifies whether a foreign DNA is inserted or not after cloning? (4)
1. Diagram shows a typical agarose gel showing migration of DNA fragments.
a. Which of the bands has largest and smallest DNA fragments?
b. How can you make fragments of DNA for electrophoresis?
c. Explain separation of DNA fragments using electrophoresis.
d. Point out a method to visualize the separated DNA fragments after electrophoresis. (4)
1. The picture given below shows the technique used for generating multiple copies of the gene of interest.
a. What is the technique called?
b. Name the reactions at Step I, Step II, Step III.
c. Explain the principle underlying this technique of DNA amplification. (4)
The above picture shows cloning vector pBR322.
a. What is ori? Give its importance.
b. How does the insertion of foreign DNA at Bam HI site selected? What is ampR?
c. How many cloning sites are depicted in this vector as shown in the figure? (4)
1. In a class room seminar on cloning vectors, your friend asked to explain the steps to introduce the plasmid DNA to a bacteria cell, Microinjection and bilestics. Answer his questions. (4)
1. Restriction endonucleases are the enzymes used to cut the DNA molecules.
a. Give the general terms for the specific sequences where these enzymes cut the DNA.
b. Name the enzyme that joins the foreign DNA and vector DNA.
c. Give any two procedures to introduce the recombinant DNA into the host cell. (4)
During genetic engineering Vector with foreign DNA is transferred into a host bacterium. The next target will be the selection of transformants from non-transformants. How antibiotic resistance and insertional inactivation is exploited for this purpose? (4)
1. While studying nucleotide sequence, Raj found that following sequence which can be recognized by some enzymes: (4)
5’ – GAATTC – 3’
3’ – CTTAAG – 5’
a. Give salient features of this sequence.
b. Write name of enzymes which recognize such sequences.
c. Elaborate importance of this enzyme in Genetic engineering.
A group of students came to know about recombinant DNA technology. They want to know how scientists can produce a new desired product using rDNA technology. Can you give them an idea about the important steps that are involved in this process? (4)