BIOTECHNOLOGY- PRINCIPLES AND PROCESSES
2016 MARCH
1.
Observe the sketch of stirred-tank bioreactor and label the parts
A, B, C and D. (Scores: 2)
1.
Manipulating
with nucleic acid is a trend in Biotechnology.
a)
Name any
one organism used as vector.
b)
What is
DNA polymerase? (Scores: 2)
2016 SAY
Electrophoresis is a method commonly used in Biotechnology. Write briefly
about Gel Electrophoresis. (2)
1.
Genetic engineering is a promising branch recently
developed in biological science.
a.
Expand PCR and name three steps in each cycle. (Scores:
2)
b.
What is a plasmid? Name three features required for
cloning vectors. (Scores:
2)
MARCH 2017
1. Sequences of base pairs in DNA that reads
the same on both the strands when the orientation of reading is kept the same are
called …………… sequences. (Score: 1)
2. (A) The following photograph shows the
result of a technique showing the separation of DNA.
a. Name the technique.
b. How the separated DNA is visualized?
c. DNA fragments of size 500 bp, 1600 bp and
2000 bp are separated by this process. Which fragment will migrate fast? Why?
‘P (Scores: 1 X 3 = 3)
OR
(B)
Different methods have been suggested to introduce alien DNA into host
cells. Give and explain any three methods adopted for this purpose. (Scores: 1
X 3 = 3)
MARCH 2018
1.
The DNA
fragments can be separated using gel electrophoresis.
a.
Name the
gel used in this technique.
b.
Write the
name of technique used to remove the DNA from the gel.
2.
Observe
the following figure.
a.
Identify
the figure.
b.
What does
(i) tetR (ii) rop (iii) ori denote?